chromium nuclei isolation kit lysis buffer (10X Genomics)

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10X Genomics chromium nuclei isolation kit lysis buffer
Chromium Nuclei Isolation Kit Lysis Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kit+buffer/pm42265312-272-6-4
Average 86 stars, based on 1 article reviews

chromium nuclei isolation kit lysis buffer - by Bioz Stars, 2026-10

86/100 stars

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Isolation:

Article Title: Dissecting inter-focal heterogeneity and convergent immunosuppressive ecosystems in multifocal hepatocellular carcinoma through single-cell and spatial multi-omics
Article Snippet: .. Nuclei isolated from tumor and adjacent normal tissues were resuspended in Nuclei Buffer (10x Genomics, Chromium Single Cell ATAC Reagent Kit). ..

Article Title: Spatially resolved single-cell analyses of human meningioma identify novel cell states influencing tumor microenvironment and progression.
Article Snippet: .. Nuclei were isolated using 10x Genomics Chromium Nuclei Isolation Kit lysis buffer with Miltenyi Anti-Nucleus beads and manual dissociation. ..

Article Title: APOE4–Aβ synergy drives brain network dysfunction and neuronal lysosomal-ER proteostasis dysregulation a preclinical Alzheimer’s disease model
Article Snippet: .. Tissue was immediately transferred to ice-cold PBS and mechanically homogenized using loose and tight Dounce pestles in Nuclei Isolation Buffer (10x Genomics, Chromium Fixed RNA Profiling Kit; CG000478) supplemented with 1% RNase inhibitor. ..

Single Cell:

Article Title: Generation of antigen-specific paired-chain antibodies using large language models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-human IgG-HRP Thermo Fisher Cat # A18817; RRID: AB_2535594 Mouse anti-RSV F (MCA490) Bio-Rad Cat# MCA490; RRID:AB_2231368 S309 PMID: 32422645 PBD: 6WS6 VRC01 BEI Resources ARP-12033; RRID: AB_2491019 CR9114 PMID: 22878502 GenBank: JX213639, JX213640 MEDI8852 PMID: 27453466 RRID: AB_3111586 5-1 Abu-Shmais et al. 35 N/A APC-Cy7 Mouse Anti-Human CD14 BD Cat# 561709; RRID: AB_10893806 FITC Anti-Human CD3 (OKT3) Tonbo Biosciences Cat# 35–0037; RRID: AB_2621662 BV711 Mouse Anti-Human CD19 BD Cat# 563036; RRID: AB_2737968 PE-Cy5 Mouse Anti-Human IgG BD Cat# 551497; RRID: AB_394220 Bacterial and virus strains RSV A2 strain BEI Resources NR-52018 SARS-CoV-2 index pseudovirus Daniel Sheward N/A SARS-CoV-1 pseudovirus Daniel Sheward N/A SARS-CoV-2 Delta pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 Gamma pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 BA.2.75.2 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 B.1 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 BJ.1 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 BA.2 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 BA.5 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 BQ.1.1 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 JN.1 pseudotyped lentivirus Daniel Sheward N/A SARS-CoV-2 XBB.1.5 pseudotyped lentivirus Daniel Sheward N/A H5N1 A/Texas/37/2024 Rebecca A. Gillespie N/A H5N1 A/Vietnam/1203/2004 Rebecca A. Gillespie N/A H1N1 A/Michigan/45/2015 Rebecca A. Gillespie N/A H5N1 A/Cambodia/2023 Rebecca A. Gillespie N/A H5N2 A/Mexico/2024 Rebecca A. Gillespie N/A H2N2 A/Singapore/1/57 Rebecca A. Gillespie N/A Biological samples Human PBMCs (healthy) StemCell Technologies Cat# 70025 Human PBMCs (HIV-1+) Mark Connors N/A Human PBMCs (COVID-19 convalescent) Helen Chu N/A Human PBMCs (Influenza vaccinated) Ted Ross N/A Chemicals, peptides, and recombinant proteins Polyethyleniminine Linear MW 25000 Polysciences Cat#23966-1 1-Step Ultra TMB-ELISA Substrate Solution Thermo Fisher Cat#34029 Pluronic Acid F-68 Fisher Cat# 24040-032 Bovine serum albumin (BSA) Sigma-Aldrich A1470 Ghost dye red 780 Tonbo biosciences Cat#13-0865 4 mM L-glutamine Fisher Cat# 25030-081 Protein A resin GenScript Cat#L00210 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, December 11, 2025 e1 Please cite this article in press as: Wasdin et al., Generation of antigen-specific paired-chain antibodies using large language models, Cell (2025), https://doi.org/10.1016/j.cell.2025.10.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Lyra-CoV Spike Wall et al. 31 N/A SARS-CoV-2 Index RBD Sino Biological Cat# 40592-VNAH SARS-CoV-2 HexaPro Index Spike PMID: 32703906 N/A SARS-CoV-1 S Jason McLellan N/A HCoV-OC43 S Jason McLellan N/A HCoV-HKU1-S-2P PMID: 28807998 N/A RSV A2 DS-Cav1 prefusion F Jason McLellan N/A RSV B9320 DS-Cav1 prefusion F Jason McLellan N/A RSV post-fusion Jason McLellan N/A hMPV F A1(NL/1/00) Jason McLellan N/A hMPV F B2(TN99-419) Jason McLellan N/A hMPV A postfusion F Jason McLellan N/A Parainfluenza virus type 3 prefusion stabilized F ectodomain PMID: 30420505 PDB: 6MJZ H5/TX/24 hemagglutinin Genscript GenBank PP577943.1 H3 HK68 Masaru Kanekiyo N/A H3 Perth19 Masaru Kanekiyo N/A H1 MI15 Masaru Kanekiyo N/A H1 NC99 Masaru Kanekiyo N/A B2 SG57 Masaru Kanekiyo N/A HA B/Wash/19 Masaru Kanekiyo N/A H5 VN04 Masaru Kanekiyo N/A H5 IN05 Masaru Kanekiyo N/A H7 Anh13 Masaru Kanekiyo N/A H5 HK09 Masaru Kanekiyo N/A H10 JD13 Masaru Kanekiyo N/A HIV-1 gp140 SOSIP BG505 Ivelin Georgiev N/A HIV-1 ZM197 env Ivelin Georgiev N/A HIV-1 B4.1 env Ivelin Georgiev N/A Norovirus CHDC P domain Grant Hansman N/A Norovirus SYD_2012 P domain Grant Hansman N/A Norovirus GII.17 P domain Grant Hansman N/A Critical commercial assays B cell Single Cell V(D)J solution 10X Genomics N/A ExpiFectamineTM 293 Transfection Kit Thermo Fisher Cat #A14526 EZ-link Sulfo-NHS-Biotin No-Weigh Thermo Fisher Cat #A39258 Solulink protein-oligonucleotide conjugation kit TriLink Biotechnologies Cat#S-9011 Plasmid Kits for Plasmid DNA Extraction QIAGEN Cat#12165 StrepTrap XT prepacked chromatography column Cytiva Cat#29401322 Deposited data Raw next-generation sequencing data This paper SRA: PRJNA1321287 Cryo-EM structure RSV-3301 and RSV-2245 fabs bound to RSV-A F This paper PDB: 9MKN, EMDB: EMD-48331 Experimental models: Cell lines Human: Expi293F cells Thermo Fisher Cat#A14527 HEK293T ATCC CRL-3216 Vero cells ATCC CCL-81 E. coli DH5α Cell Culture Core N/A (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e8, December 11, 2025 Please cite this article in press as: Wasdin et al., Generation of antigen-specific paired-chain antibodies using large language models, Cell (2025), https://doi.org/10.1016/j.cell.2025.10.006 Article .. Cell lines used in this study include Expi293F (Thermo Fisher), HEK293T (ATCC), and Vero cells (ATCC).

Article Title: Venezuelan equine encephalitis virus infection causes chronic neurobehavioral outcomes, cellular remodeling, and hippocampal single-cell transcriptomic changes.
Article Snippet: .. From the right hemisphere, the hippocampus was dissected and placed into 10x genomics fixation buffer solution (Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit #1000414, 10x Genomics, Pleasanton, CA). ..

Article Title: Loss of MITF activity leads to emergent cell states from the melanocyte stem cell lineage
Article Snippet: Fluorescent cells were collected in 100μl of 0.04% Bovine Serum Albumin (BSA)/PBS in DNA-LoBind tubes (Fisher Scientific), spun down at 300G at 4°C, resuspended in 34 μl of 0.04% BSA/PBS, and immediately processed using the Chromium platform (10x Genomics) with one lane per sample. .. Single-cell mRNA libraries were prepared using either the single-cell 3’ solution V2 kit (10x Genomics) – embryos or the single-cell 3’ solution V3.1 kit (10x Genomics) – metamorphic fish. .. Quality control and quantification assays were performed using High Sensitivity DNA kits on a Bioanalyzer (Agilent).

Concentration Assay:

Article Title: Protocol to maximize single-nuclei multiome library complexity for limited primary tissues via optimized sample preparation and library synthesis.
Article Snippet: .. Reagent Final concentration Volume added Nuclease free water NA 45.75 μl 203 nuclei buffer (10X Genomics kit) 13 2.5 μl 100mM DTT 1mM 0.5 μl 40U/μl RNase inhibitor 1U/μl 1.25 μl Keep the freshly prepared nuclei suspension buffer on ice and use within 1 hour. ..

Suspension:

Virus:

Transfection:

Conjugation Assay:

Plasmid Preparation:

DNA Extraction:

Chromatography:

Next-Generation Sequencing:

Cryo-EM Sample Prep:

Cell Culture:

Sample Prep:

other:

Article Title: TNF-mediated hilar interneuron loss and aberrant granule cell migration are associated with chronic cognitive deficits following TBI.
Article Snippet: All measurements were performed using an xMAP 465 instrument (Luminex, Austin, TX).

Review